《植物生理学报》 2009, 45(6): 579-582

低温预处理对水曲柳体细胞胚胎发生的影响

李楠^1,2, 孔冬梅^2,3, 沈海龙^1,2,*

1 东北林业大学林学院, 哈尔滨 150040; 2 东北林业大学林木遗传育种与生物技术教育部重点实验室, 哈尔滨 150040; 3 山西 大学生命科学与技术学院, 太原 030006

通信作者：沈海龙；E-mail: shenhl-cf@nefu.edu.cn；Tel: 0451-82191044

摘　要：

用4 ℃低温预处理未成熟的水曲柳种子0~30 d, 取出种子内的合子胚为外植体诱导体胚发生, 研究低温预处理影响体 胚发生的结果表明: 低温预处理过的外植体其体胚发生总数和子叶胚发生数均低于未作低温预处理的; 随着预处理时间的 延长, 畸形胚发生数和发生比率与总的体胚发生数和发生率的变化趋势基本相同; 处理20 d的正常胚发生数和发生比率的 绝对数虽然很低, 但远高于不作低温预处理的。说明4 ℃低温预处理对水曲柳体胚发生没有促进作用, 对畸形胚的发生也 不能控制, 总的来讲, 适当的低温处理有一定的改善正常体胚发生的潜力。

关键词：水曲柳; 低温预处理; 体胚发生; 畸形胚

收稿：2009-02-23   修定：2009-05-20

资助：国家自然科学基金(30170772)。

Effect of Low Temperature Pretreatment on Somatic Embryogenesis of Fraxinus mandshurica Rupr.

LI Nan^1,2, KONG Dong-Mei^2,3, SHEN Hai-Long^1,2,*

¹School of Forestry, Northeast Forestry University, Harbin 150040, China; ²Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, China; ³College of Life Science and Technology, Shanxi University, Taiyuan, Shanxi 030006, China

Corresponding author: SHEN Hai-Long; E-mail: shenhl-cf@nefu.edu.cn; Tel: 0451-82191044

Abstract:

The effects of low temperature pretreatment on somatic embryogenesis of Fraxinus mandshurica were investigated by using zygotic embryos from immature seeds that pretreated under 4 ℃ for 0–30 d as explants. The results showed that the total somatic embryos and cotyledonary somatic embryos induced from the pretreated explants were lower than those of control; the fluctuation trends of abnormal somatic embryos and their ratio to total somatic embryos along with the pretreatment time changes were similar to those of total somatic embryos and induction ratio; however, although the absolute normal somatic embryos and its ratio to total somatic embryos were very low in the 20 d treatment, they were much higher than those of control. This indicated that 4 ℃ low temperature pretreatment had no improvement effect on F. mandshurica somatic embryogenesis and abnormal somatic embryo control, but the proper treatment had the potential to improve normal somatic embryogenesis.

Key words: Fraxinus mandshurica; low temperature pretreatment; somatic embryogenesis; abnormal somatic embryos